Kentucky Route 101 is a north–south highway traversing three counties in South Central Kentucky.

KY 101 starts at a junction with KY 259 near the community of Rhoda in Edmonson County.

A little over four miles (6 km) from its beginning, KY 101 passes into Warren County. It cojoins with U.S. 31-W for less than a mile before splitting on a southern course that takes it through the center of Smiths Grove. Just south of Smiths Grove, KY 101 intersects with I-65 at the Exit 38 interchange.

KY 101 then continues on for less than a mile before intersecting with the cojoined U.S. 68/KY 80.

Shortly after passing through the Three Forks community, KY 101 passes into Allen County. The route intersects with U.S. 31-E, just north of Scottsville before reaching its terminus at KY 100 near downtown.