State Route 727 (SR 727, OH 727) is a north–south state highway located in the southwestern portion of the U.S. state of Ohio. State Route 727 has its southern terminus at a T-intersection with State Route 131 approximately 1.50 miles (2.41 km) west of Newtonsville. The highway's northern terminus is at a T-intersection with State Route 133 nearly 5.25 miles (8.45 km) southwest of Blanchester.

All of State Route 727 is nestled within the northeastern corner of Clermont County. This route is not included as a component of the National Highway System, a network of routes determined to be the most important for the economy, mobility and defense of the nation.

State Route 727 was established in 1937. It has maintained the same northeastern Clermont County routing between State Route 131 and State Route 133 from its inception to the present day. State Route 727 has not experienced any major changes since making its debut.

The entire route is in Clermont County.